| 1 | Ellen van Vroonhoven (Senior) | NED | 45.08 | | - |
| 2 | Anouk van Westerhoven (Neo) | NED | 45.78 | | +0.700 |
| 3 | Tosca Versloot (B2) | NED | 46.33 | | +1.250 |
| 4 | Hiltje Gerritsma (B2) | NED | 49.81 | | +4.730 |
| 5 | Renee van Boekel (Neo) | NED | 50.43 | | +5.350 |
| 6 | Hanneke Goud (A1) | NED | 51.37 | | +6.290 |
| 7 | Jasmijn Hiddink (D1) | NED | 51.51 | | +6.430 |
| 8 | Justine Eerden (Senior) | NED | 51.68 | | +6.600 |
| 9 | Ciska Koole (M40) | NED | 51.76 | | +6.680 |
| 10 | Alina van de Kracht (B2) | NED | 52.19 | SB | +7.110 |
| 11 | Hanneke Reincke (A1) | NED | 52.26 | | +7.180 |
| 12 | Kadika Dijkstra-Hoefsloot (C2) | NED | 53.83 | | +8.750 |
| 13 | Arlieke Teunisse (Senior) | NED | 53.93 | | +8.850 |
| 14 | Eline Bekkers (Neo) | NED | 54.08 | | +9.000 |
| 15 | Lisa Kuiper (B1) | NED | 55.09 | | +10.010 |
| 16 | Paulien Abma (C2) | NED | 56.54 | | +11.460 |
| 17 | Evie van der Wijst (Senior) | NED | 56.69 | | +11.610 |
| 18 | Esther van den Boogaard (B2) | NED | 58.24 | | +13.160 |
| 19 | Melle Boeijen (E2) | NED | 1:01.59 | | +16.510 |
| 20 | Floor van Schijndel (E2) | NED | 1:07.01 | | +21.930 |
| 21 | Sophie van Hout (D1) | NED | 1:07.57 | | +22.490 |
| DNS | Alisha Hansma (A2) | NED | DNS | | - |