| 1 | Ymke Wubben (B1) | NED | 44.80 | | - |
| 2 | Hilde Houtzager (Neo) | NED | 44.87 | SB | +0.070 |
| 3 | Marit Dee (Neo) | NED | 48.18 | | +3.380 |
| 4 | Keira Vissenberg (C2) | NED | 48.28 | | +3.480 |
| 5 | Simone Gravesteijn (B1) | NED | 48.37 | | +3.570 |
| 6 | Meike van Veen (Neo) | NED | 48.44 | | +3.640 |
| 7 | Emma Boon (C2) | NED | 48.56 | | +3.760 |
| 8 | Gwen Wubben (A2) | NED | 49.60 | SB | +4.800 |
| 9 | Amanda van Leeuwen (A1) | NED | 52.29 | | +7.490 |
| 10 | Guusje Zautsen (Neo) | NED | 52.73 | | +7.930 |
| 11 | Balou Nelck (Neo) | NED | 53.20 | | +8.400 |
| 12 | Carolien van der Goes (n/a) | NED | 54.96 | SB | +10.160 |
| 13 | Natascha Schoenmaker (n/a) | NED | 56.94 | PB | +12.140 |
| 14 | Anja Slingerland (n/a) | NED | 57.79 | SB | +12.990 |
| 15 | Sun Dong Mollema (n/a) | NED | 58.74 | | +13.940 |
| 16 | Fenna Vissenberg (D1) | NED | 59.27 | | +14.470 |
| 17 | Karien ter Welle (n/a) | NED | 59.56 | | +14.760 |
| 18 | Floor Eeken (Neo) | NED | 1:00.08 | PB | +15.280 |
| 19 | Laura Gravesteyn (D2) | NED | 1:01.67 | SB | +16.870 |
| 20 | Danique Pera (B2) | NED | 1:02.85 | SB | +18.050 |
| 21 | Hannah Bervoets (n/a) | NED | 1:03.27 | PB | +18.470 |
| 22 | Lisa van der Goes (D1) | NED | 1:03.52 | | +18.720 |
| 23 | Elsemiek Dikker (Senior) | NED | 1:06.76 | | +21.960 |
| 24 | Sophie Keemink (Senior) | NED | 1:06.81 | SB | +22.010 |
| 25 | Elena Kwee (D2) | NED | 1:07.43 | PB | +22.630 |
| 26 | Linda Boon (n/a) | NED | 1:07.95 | PB | +23.150 |
| 27 | Beatrijs Hinloopen (n/a) | NED | 1:08.14 | PB | +23.340 |
| 28 | Lisa Elzinga (Senior) | NED | 1:08.33 | | +23.530 |
| 29 | Rosalie Tol (Neo) | NED | 1:09.22 | | +24.420 |
| 30 | Puck Breedijk (D2) | NED | 1:09.93 | PB | +25.130 |
| 31 | Daniek van der Kaaij (n/a) | NED | 1:10.91 | PB | +26.110 |
| 32 | Marjolijn Hobo (n/a) | NED | 1:12.88 | PB | +28.080 |
| 33 | Koen de-Li Roelofs (C1) | NED | 1:13.88 | | +29.080 |
| 34 | Meike Spanbroek (D1) | NED | 1:18.40 | | +33.600 |
| DNS | Maaike Elgersma (Senior) | NED | DNS | | - |
| DNS | Rozemarijn Ammerlaan (n/a) | NED | DNS | | - |