| 1 | Petra de Boer-Grimbergen (Senior) | NED | 48.74 | SB | - |
| 2 | Amy Jonk (B1) | NED | 49.06 | SB | +0.320 |
| 3 | Saskia Maarse (Neo) | NED | 50.20 | SB | +1.460 |
| 4 | Corinne Geertjens Huiber (Senior) | NED | 51.58 | SB | +2.840 |
| 5 | Daphne Teune (A1) | NED | 51.67 | SB | +2.930 |
| 6 | Henriëtte Goede-Hartog (M30) | NED | 51.75 | | +3.010 |
| 7 | Rosalie Grim (C1) | NED | 51.83 | SB | +3.090 |
| 8 | Astrid Wegman (A1) | NED | 52.65 | SB | +3.910 |
| 9 | Marjan Muis (M30) | NED | 54.31 | SB | +5.570 |
| 10 | Laura Temme (Senior) | NED | 54.82 | SB | +6.080 |
| 11 | Ingrid Wegman (D2) | NED | 55.51 | SB | +6.770 |
| 12 | Diane Comello (Senior) | NED | 56.20 | SB | +7.460 |
| 13 | Reineke Schoot (A1) | NED | 57.76 | PB | +9.020 |
| 14 | Joyce van de Berg (B2) | NED | 57.86 | SB | +9.120 |
| 15 | Erica van Beek (Senior) | NED | 58.37 | SB | +9.630 |
| 16 | Petra van der Weijden (B2) | NED | 1:00.80 | SB | +12.060 |
| 17 | Rosa Hoogstraten (D2) | NED | 1:01.01 | SB | +12.270 |
| 18 | Sophie van de Veldt (M40) | NED | 1:03.07 | SB | +14.330 |
| 19 | Inge Brakman (M35) | NED | 1:05.61 | SB | +16.870 |
| 20 | Charlotte Stam (D2) | NED | 1:08.58 | SB | +19.840 |