| 1 | Naomi Kammeraat (C2) | NED | 42.83 | | - |
| 2 | Ankie Ytsma (M35) | NED | 43.63 | SB | +0.800 |
| 3 | Nynke Ytje Boonstra (A1) | NED | 43.90 | | +1.070 |
| 4 | Rosa Lieke Smits (B2) | NED | 45.87 | SB | +3.040 |
| 5 | Roos de Vries (A1) | NED | 46.76 | | +3.930 |
| 6 | Lieke.0807 Visser (C2) | NED | 47.22 | | +4.390 |
| 7 | Jenieke Kuiken (A1) | NED | 47.59 | | +4.760 |
| 8 | Femke Boskma (B1) | NED | 48.39 | SB | +5.560 |
| 9 | Jelly Hiemstra (B1) | NED | 48.47 | | +5.640 |
| 10 | Jildau de Vries (D2) | NED | 48.88 | | +6.050 |
| 11 | Jolijn Kooistra-de Vries (M35) | NED | 48.97 | SB | +6.140 |
| 12 | Jennifer Buis (Neo) | NED | 49.65 | | +6.820 |
| 13 | Silke Ludema (B1) | NED | 49.84 | | +7.010 |
| 14 | Sandra van der Heide (B1) | NED | 50.05 | SB | +7.220 |
| 15 | Antje Ypma (D1) | NED | 50.79 | | +7.960 |
| 16 | Gesina De Vries (A1) | NED | 50.86 | PB | +8.030 |
| 17 | Doutsen de Vries (B1) | NED | 50.90 | PB | +8.070 |
| 18 | Miriam Gossen (Neo) | NED | 50.95 | | +8.120 |
| 19 | Geertje de Boer (B2) | NED | 51.20 | SB | +8.370 |
| 20 | Nina Kuiken (B1) | NED | 51.33 | | +8.500 |
| 21 | Barbara Heerschop (M65) | NED | 51.38 | | +8.550 |
| 22 | Julia Brolsma (D2) | NED | 51.81 | | +8.980 |
| 23 | Tessa Tasma (C2) | NED | 52.26 | | +9.430 |
| 24 | Thirza De Vries (B1) | NED | 52.64 | SB | +9.810 |
| 25 | Juul Plomp (D2) | NED | 53.96 | SB | +11.130 |
| 26 | Welmoed de Boer (D2) | NED | 54.29 | | +11.460 |
| 27 | Karlijn Westra (D1) | NED | 54.70 | | +11.870 |
| 28 | Marije Hiemstra (A2) | NED | 55.06 | PB | +12.230 |
| 29 | Doutzen Kingma (C1) | NED | 55.26 | | +12.430 |
| 30 | Nima Bergsma (A1) | NED | 55.92 | PB | +13.090 |
| 31 | Fenna Bosma (C2) | NED | 57.20 | SB | +14.370 |
| 32 | Susan Dijkshoorn (Senior) | NED | 57.75 | | +14.920 |
| 33 | Jasmine de Vries (B1) | NED | 58.56 | PB | +15.730 |
| 34 | Caroline Giesberts (M60) | NED | 58.84 | SB | +16.010 |
| 35 | Else Marie Boersma (D2) | NED | 58.99 | | +16.160 |
| 36 | Femke Posthuma (D1) | NED | 1:01.89 | SB | +19.060 |
| 37 | Mariëtte Hees (M60) | NED | 1:04.23 | SB | +21.400 |
| 38 | Roelien Linthorst (Senior) | NED | 1:04.41 | SB | +21.580 |
| 39 | Marith Van Der Tuin (D2) | NED | 1:06.97 | PB | +24.140 |
| 40 | Laurien Lootsma (D2) | NED | 1:07.23 | PB | +24.400 |
| 41 | Afke Brandenburg (M55) | NED | 1:09.69 | SB | +26.860 |