| 1 | Jeike Juk (Neo) | NED | 48.81 | | - |
| 2 | Jenny van der Velde (Senior) | NED | 49.10 | SB | +0.290 |
| 3 | Judith Loopers (Senior) | NED | 54.35 | | +5.540 |
| 4 | Saskia van der Velde (Senior) | NED | 54.83 | | +6.020 |
| 5 | Marjet Janssen (Senior) | NED | 56.33 | | +7.520 |
| 6 | Fleur Mollema (Neo) | NED | 57.36 | | +8.550 |
| 7 | Fimke Anna Giliam (Neo) | NED | 59.89 | | +11.080 |
| 8 | Klaske Terpstra (Neo) | NED | 1:00.65 | PB | +11.840 |
| 9 | Isabel Stribos (Senior) | NED | 1:00.84 | | +12.030 |
| 10 | Lotte Tenkink (Neo) | NED | 1:03.06 | | +14.250 |
| 11 | Lisanne van der Werf (Neo) | NED | 1:04.68 | | +15.870 |
| 12 | Adrianne de Roo (Neo) | NED | 1:06.87 | | +18.060 |
| 13 | Amarins Jager (Senior) | NED | 1:07.05 | | +18.240 |
| 14 | Iris van der Vegte (Senior) | NED | 1:16.64 | | +27.830 |
| 15 | Femke Salverda (Senior) | NED | 1:20.29 | | +31.480 |
| 16 | Merel Hage (Senior) | NED | 1:21.02 | | +32.210 |
| DNF | Hedwig Fossen (Neo) | NED | DNF | | - |
| DNF | Anne Blanken (Senior) | NED | DNF | | - |
| DQ | Nynke Ant Annema (Senior) | NED | DQ | | - |