| 1 | Kimi Goetz (Senior) | USA | 37.76 | | - |
| 2 | McKenzie Browne (Senior) | USA | 38.87 | | +1.110 |
| 3 | Sarah Warren (Senior) | USA | 39.18 | | +1.420 |
| 4 | Greta Myers (Neo) | USA | 39.37 | | +1.610 |
| 5 | Sofia Prosvirnova (Senior) | DEN | 40.29 | NR | +2.530 |
| 6 | Mia Manganello (Senior) | USA | 40.46 | | +2.700 |
| 7 | Anna Quinn (Senior) | USA | 40.80 | | +3.040 |
| 8 | Giorgia Birkeland (Neo) | USA | 41.71 | | +3.950 |
| 9 | Laura Michelle Rodriguez (A1) | COL | 41.81 | | +4.050 |
| 10 | Lindsey Woodbury (Neo) | USA | 42.07 | | +4.310 |
| 11 | Libby Williams (A2) | USA | 42.25 | | +4.490 |
| 12 | Ella Teeples (C2) | USA | 42.66 | PB | +4.900 |
| 13 | Isabela Vargas Rodriguez (B2) | COL | 42.91 | | +5.150 |
| 14 | Marley Soldan (B2) | USA | 42.97 | | +5.210 |
| 15 | Abigél Mercs (Neo) | HUN | 43.41 | | +5.650 |
| 16 | Melissa Dahlmann (M40) | USA | 43.77 | | +6.010 |
| 17 | Karen Lizeth Siatama Valencia (A1) | COL | 44.03 | | +6.270 |
| 18 | Emma de Bock (C2) | USA | 45.08 | | +7.320 |
| 19 | Rz Love (C2) | USA | 45.87 | | +8.110 |
| 20 | Clare Jeong (Senior) | USA | 46.26 | | +8.500 |
| 21 | Kaitlyn Lee (C2) | USA | 46.37 | | +8.610 |
| 22 | Leah Choi (B1) | USA | 46.65 | | +8.890 |
| 23 | Finnley D'Card (C2) | USA | 47.61 | PB | +9.850 |
| 24 | Audrey Han (B1) | USA | 47.90 | | +10.140 |
| 25 | Mariana Acero Pineda (C1) | COL | 48.59 | | +10.830 |
| 26 | Elisha Lee (D2) | USA | 48.65 | SB | +10.890 |
| 27 | Scarelette Gramlich Thatcher (D2) | USA | 49.58 | SB | +11.820 |
| 28 | Allison La (D2) | USA | 50.92 | | +13.160 |
| 29 | Juliette Song (D2) | USA | 51.22 | | +13.460 |
| 30 | Kaylin La (D2) | USA | 51.93 | SB | +14.170 |
| 31 | Baily Luck (Senior) | USA | 53.56 | SB | +15.800 |
| 32 | Atira Meacham (E1) | USA | 1:04.25 | SB | +26.490 |