| 1 | Christine Aaftink (Neo) | NED | 41.34 | | - |
| 2 | Herma Emmens-Meijer (Neo) | NED | 41.58 | | +0.240 |
| 3 | Sandra Voetelink (A2) | NED | 41.69 | NRJ | +0.350 |
| 4 | Anita Loorbach (Senior) | NED | 42.05 | | +0.710 |
| 5 | Tineke Hulzebosch (Neo) | NED | 42.15 | SB | +0.810 |
| 6 | Marieke Stam (Senior) | NED | 42.70 | | +1.360 |
| 7 | Sandra Zwolle (A2) | NED | 42.87 | SB | +1.530 |
| 8 | Jacqueline van Straaten (Senior) | NED | 42.93 | PB | +1.590 |
| 9 | Annamarie Thomas (A1) | NED | 42.98 | SB | +1.640 |
| 10 | Jolanda Grimbergen (Neo) | NED | 43.04 | | +1.700 |
| 11 | Alida Pasveer (M30) | NED | 43.20 | SB | +1.860 |
| 12 | Marga Preuter (Neo) | NED | 43.27 | | +1.930 |
| 13 | Renske Vellinga (C2) | NED | 43.33 | NRJ | +1.990 |
| 14 | Ellen Linnenbank (B2) | NED | 43.41 | | +2.070 |
| 15 | Lia van Schie (A2) | NED | 43.74 | | +2.400 |
| 16 | Henriët van der Meer (Neo) | NED | 43.79 | SB | +2.450 |
| 17 | Carla Zijlstra (Neo) | NED | 44.09 | SB | +2.750 |
| 18 | Hanneke de Vries (Senior) | NED | 44.44 | | +3.100 |