| 1 | Elisabeth Baier (B2) | GER | 42.87 | | - |
| 2 | Anna Ostlender (C2) | GER | 43.08 | | +0.210 |
| 3 | Kristin Meyer (B1) | GER | 43.34 | | +0.470 |
| 4 | Joelle Günther (B2) | GER | 43.57 | | +0.700 |
| 5 | Katharina Pönisch (C2) | GER | 43.73 | | +0.860 |
| 6 | Christl Anders (B2) | GER | 43.79 | | +0.920 |
| Meghan Wystrichowski (C2) | GER | 43.79 | | +0.920 |
| 8 | Gianina Künstler (C2) | GER | 44.22 | | +1.350 |
| 9 | Babett Unger (C1) | GER | 44.43 | | +1.560 |
| 10 | Sylvie Zehmisch (Neo) | GER | 44.46 | | +1.590 |
| 11 | Henrike Michalke (B1) | GER | 44.97 | | +2.100 |
| 12 | Vanessa Nana Niznik (B1) | GER | 45.34 | | +2.470 |
| 13 | Olivia Kattner (D2) | GER | 45.79 | SB | +2.920 |
| 14 | Caroline Möller (C1) | GER | 45.89 | | +3.020 |
| 15 | Lucy Mehnert (C2) | GER | 45.98 | | +3.110 |
| 16 | Emma Fink (A2) | USA | 46.08 | SB | +3.210 |
| 17 | Maja Metz (C2) | GER | 46.17 | | +3.300 |
| 18 | Melody Igrishta (C1) | GER | 46.82 | | +3.950 |
| 19 | Jana Lunze (D2) | GER | 46.96 | | +4.090 |
| 20 | Erika Broklová (C2) | CZE | 47.22 | | +4.350 |
| 21 | Marlene Zink (D2) | GER | 47.34 | | +4.470 |
| 22 | Cheyenne Schmidt (C1) | GER | 47.36 | | +4.490 |
| 23 | Franziska Fiebig (D2) | GER | 47.95 | SB | +5.080 |
| 24 | Jessica Fiebig (C2) | GER | 48.28 | PB | +5.410 |
| 25 | Antonia Luisa Rückmann (D2) | GER | 48.71 | | +5.840 |
| 26 | Adela Petrusova (D2) | CZE | 49.97 | | +7.100 |
| 27 | Gabriela Beranova (C2) | CZE | 50.09 | PB | +7.220 |
| 28 | Vanessa Wöhner (C1) | GER | 51.27 | | +8.400 |
| 29 | Theresa Spörl (D1) | GER | 52.00 | | +9.130 |
| 30 | Eliska Fackenbergova (E2) | CZE | 53.07 | | +10.200 |
| 31 | Alisa Korolyova (D2) | GER | 54.28 | SB | +11.410 |
| 32 | Petra Zhorová (D1) | CZE | 54.57 | | +11.700 |
| 33 | Eliska Hunackova (E1) | CZE | 55.74 | | +12.870 |
| 34 | Adela Simnova (D1) | CZE | 58.50 | | +15.630 |
| 35 | Linette-Elin Rückmann (E2) | GER | 1:03.24 | | +20.370 |
| 36 | Amálie Doláková (F1) | CZE | 1:07.23 | PB | +24.360 |
| DNF | Christine Seemann (M45) | GER | DNF | | - |