| 1 | Nikola Zdráhalova (Senior) | CZE | 39.07 | SB | - |
| 2 | Mihaela Hogas (Neo) | ROU | 39.25 | | +0.180 |
| 3 | Katja Franzen (M30) | GER | 39.49 | | +0.420 |
| 4 | Anna Ostlender (A2) | GER | 39.58 | | +0.510 |
| 5 | Martina Sáblíková (Senior) | CZE | 39.78 | SB | +0.710 |
| 6 | Josephine Heimerl (Neo) | GER | 39.96 | | +0.890 |
| 7 | Bianca-Lorena Stănică (Neo) | ROU | 40.81 | | +1.740 |
| 8 | Laura Kivioja (A1) | FIN | 40.92 | SB | +1.850 |
| 9 | Anna Petutschnigg (A2) | AUT | 41.16 | SB | +2.090 |
| 10 | Andreea Haldan (Neo) | ROU | 41.36 | | +2.290 |
| 11 | Isabel Kraus (B1) | GER | 41.43 | | +2.360 |
| 12 | Raluca Doina Ştef (Senior) | ROU | 41.56 | | +2.490 |
| 13 | Veronika Antosova (Neo) | CZE | 41.95 | | +2.880 |
| 14 | Nikol Nepokojova (A1) | CZE | 42.00 | | +2.930 |
| 15 | Katharina Thien (Neo) | AUT | 42.46 | | +3.390 |
| 16 | Roxana-Elena Manolache (Neo) | ROU | 42.95 | | +3.880 |
| 17 | Stina Letteboer (C2) | GER | 43.12 | | +4.050 |
| 18 | Leonie Mayrhofer (B2) | GER | 43.25 | | +4.180 |
| 19 | Tilda-Sofiia Oras (Neo) | FIN | 43.27 | | +4.200 |
| 20 | Inka Koivisto (A2) | FIN | 43.37 | PB | +4.300 |
| 21 | Diana-Andreea Nastasache (A2) | ROU | 44.04 | | +4.970 |
| 22 | Veera Lehtonen (B2) | FIN | 44.16 | | +5.090 |
| 23 | Adela Petrusova (B2) | CZE | 44.37 | | +5.300 |
| 24 | Katerina Macháckova (B1) | CZE | 44.65 | SB | +5.580 |
| 25 | Julia Bachl (A1) | GER | 44.72 | | +5.650 |
| 26 | Julia Geutner (C2) | GER | 45.00 | | +5.930 |
| 27 | Hanna Müller (B2) | AUT | 45.14 | SB | +6.070 |
| 28 | Jessica Gerke (B1) | GER | 45.18 | SB | +6.110 |
| 29 | Sanni Rosendahl (B2) | FIN | 45.29 | PB | +6.220 |
| 30 | Villiina Suomalainen (B1) | FIN | 45.52 | SB | +6.450 |
| 31 | Antonia Bach (B1) | GER | 45.68 | SB | +6.610 |
| 32 | Adéla Krejčová (D2) | CZE | 47.49 | | +8.420 |
| 33 | Ivona Matkovic (D2) | AUT | 47.92 | SB | +8.850 |
| 34 | Ema Schneiderova (C2) | CZE | 49.06 | | +9.990 |
| 35 | Barbora Slámová (D2) | CZE | 49.52 | | +10.450 |
| 36 | Kristýna Němcová (C1) | CZE | 49.82 | | +10.750 |
| 37 | Lea Seppele (B1) | AUT | 50.55 | | +11.480 |
| 38 | Julia Paulmichl (Neo) | AUT | 55.48 | | +16.410 |
| 39 | Linda Parolini (D2) | AUT | 56.35 | SB | +17.280 |
| DQ | Monika Macháčková (C1) | CZE | DQ | | - |