| 1 | Lara Dingjan (Neo) | NED | 44.86 | | - |
| 2 | Milou Butter (Neo) | NED | 45.14 | | +0.280 |
| 3 | Tessa Dijksman (Senior) | NED | 46.47 | | +1.610 |
| 4 | Roosmarijn Hoekstra (Neo) | NED | 46.86 | | +2.000 |
| 5 | Lianne van Assema (B1) | NED | 46.97 | | +2.110 |
| 6 | Renske van der Veer (Neo) | NED | 46.99 | | +2.130 |
| 7 | Bente Adema (B1) | NED | 47.01 | | +2.150 |
| 8 | Evy van Duijn (B2) | NED | 47.25 | | +2.390 |
| 9 | Aranka Keur (M40) | NED | 47.89 | | +3.030 |
| 10 | Annette Laarhoven (A1) | NED | 48.79 | | +3.930 |
| 11 | Noa Bierens (B2) | NED | 49.02 | | +4.160 |
| 12 | Charline Coates-Kwadrin (A1) | GBR | 49.03 | | +4.170 |
| 13 | Ciska de Ree (M35) | NED | 49.33 | | +4.470 |
| 14 | Willemijn Schouwenaar (A1) | NED | 50.15 | | +5.290 |
| 15 | Sylvie van den Heuvel (Neo) | NED | 50.36 | | +5.500 |
| 16 | Anne Lubbers (B2) | NED | 50.41 | | +5.550 |
| 17 | Emma Hartveld (Neo) | NED | 50.66 | | +5.800 |
| 18 | Josine Kroon (Senior) | NED | 51.05 | | +6.190 |
| 19 | Valerie Nijman (A2) | NED | 52.43 | | +7.570 |
| 20 | Wieteke Huiberts (Neo) | NED | 52.88 | | +8.020 |
| 21 | Pien Lodder (Neo) | NED | 53.23 | | +8.370 |
| 22 | Eveline Krom (A2) | NED | 53.71 | SB | +8.850 |
| 23 | Lisanne Hartveld (B1) | NED | 54.35 | | +9.490 |
| 24 | Marijne Spijksma (B1) | NED | 55.29 | | +10.430 |
| 25 | Zoe Verhagen (B1) | NED | 56.32 | PB | +11.460 |
| 26 | Britt van Hameren (B2) | NED | 56.55 | | +11.690 |
| 27 | Mirthe Visser (B2) | NED | 57.23 | | +12.370 |
| 28 | Wendy Straathof (Senior) | NED | 57.84 | SB | +12.980 |
| 29 | Tjarda Adema-Platvoet (M45) | NED | 58.21 | | +13.350 |
| 30 | Judith van Hesselingen (M55) | NED | 58.96 | | +14.100 |
| 31 | Ramona van Leeuwen-van Noort (M55) | NED | 1:00.57 | | +15.710 |
| WDR | Kersty Heeremans (Neo) | NED | WDR | | - |