| 1 | Melanie Ooms (D2) | NED | 46.20 | SB | - |
| 2 | Anouk Kalshoven (D2) | NED | 46.84 | | +0.640 |
| 3 | Yfke Vreeken (D2) | NED | 47.44 | | +1.240 |
| 4 | Laurena van Doorn (D2) | NED | 47.92 | | +1.720 |
| 5 | Emma Tauber (D2) | NED | 51.20 | SB | +5.000 |
| 6 | Hanna van Ruiten (D1) | NED | 52.39 | SB | +6.190 |
| 7 | Floor Romme (D2) | NED | 53.39 | PB | +7.190 |
| 8 | Tara Fijten (D1) | NED | 53.68 | | +7.480 |
| 9 | Merle Adema (D1) | NED | 53.86 | SB | +7.660 |
| 10 | Kaat van den Berg (D2) | NED | 54.33 | PB | +8.130 |
| 11 | Nienke van Asten (D1) | NED | 55.04 | SB | +8.840 |
| 12 | Hille de Graaf (D2) | NED | 55.09 | | +8.890 |
| 13 | Dominique van der Aa (D2) | NED | 55.18 | PB | +8.980 |
| 14 | Fien Middelkoop (D1) | NED | 56.11 | PB | +9.910 |
| 15 | Julie Vermeulen (D2) | NED | 57.52 | PB | +11.320 |
| 16 | Lisa Mei Hartgerink (D1) | NED | 57.66 | SB | +11.460 |
| 17 | Lynn Kuipers (D2) | NED | 58.18 | PB | +11.980 |
| 18 | Isa Pruisscher (D2) | NED | 58.35 | PB | +12.150 |
| 19 | Marise Krom (D1) | NED | 59.27 | SB | +13.070 |
| 20 | Phyleine Warmenhoven (D1) | NED | 59.38 | SB | +13.180 |
| 21 | Tirza Fontaine (n/a) | NED | 1:00.70 | PB | +14.500 |
| 22 | Sarah Potters (E2) | NED | 1:02.37 | | +16.170 |
| 23 | Manou van Blijswijk (D2) | NED | 1:02.63 | PB | +16.430 |
| 24 | Iris Fijten (E2) | NED | 1:03.15 | PB | +16.950 |
| 25 | Layla Geschev (E2) | NED | 1:04.56 | SB | +18.360 |
| 26 | Kensi Tregillis-Shea (n/a) | NED | 1:04.79 | PB | +18.590 |
| 27 | Lottie Vermeulen (D1) | NED | 1:05.11 | PB | +18.910 |
| 28 | Fiene Dekker (D2) | NED | 1:05.69 | SB | +19.490 |
| 29 | Luana Zillig (n/a) | NED | 1:06.57 | PB | +20.370 |
| 30 | Kiki van Konijnenburg (n/a) | NED | 1:07.09 | PB | +20.890 |
| 31 | Noortje Nellie Stam (n/a) | NED | 1:07.53 | PB | +21.330 |
| 32 | Rika Buitelaar (D1) | NED | 1:07.93 | SB | +21.730 |
| 33 | Sanne Neijzing (D2) | NED | 1:08.06 | SB | +21.860 |
| 34 | Fiene Seijmonsbergen (E2) | NED | 1:10.22 | PB | +24.020 |
| 35 | Saar Langeslag (E2) | NED | 1:10.32 | SB | +24.120 |
| 36 | Niamh van Ombergen (E2) | NED | 1:12.10 | PB | +25.900 |
| 37 | Yfke Fopma (E2) | NED | 1:16.11 | PB | +29.910 |
| 38 | Janne Streep (E2) | NED | 1:21.97 | SB | +35.770 |