| 1 | Dione Voskamp (Neo) | NED | 39.15 | | - |
| 2 | Ireen Wüst (Senior) | NED | 39.42 | | +0.270 |
| 3 | Roxanne van Hemert (Senior) | NED | 39.43 | SB | +0.280 |
| 4 | Naomi Weeland (Neo) | NED | 39.44 | PB | +0.290 |
| Joy Beune (A2) | NED | 39.44 | | +0.290 |
| 6 | Mayon Kuipers (Senior) | NED | 39.56 | | +0.410 |
| 7 | Lotte van Beek (Senior) | NED | 39.64 | | +0.490 |
| 8 | Leeyen Harteveld (Neo) | NED | 39.70 | PB | +0.550 |
| 9 | Femke Beuling (A1) | NED | 39.73 | | +0.580 |
| 10 | Danouk Bannink (Neo) | NED | 39.84 | SB | +0.690 |
| 11 | Elisa Dul (A2) | NED | 39.97 | | +0.820 |
| 12 | Helga Drost (A2) | NED | 39.98 | | +0.830 |
| 13 | Rachelle van de Griek (A1) | NED | 40.00 | | +0.850 |
| 14 | Manouk van Tol (Senior) | NED | 40.17 | | +1.020 |
| 15 | Sanne van der Schaar (Senior) | NED | 40.18 | | +1.030 |
| 16 | Tessa Boogaard (Neo) | NED | 40.42 | | +1.270 |
| 17 | Nienke Kleinsman (Neo) | NED | 40.56 | | +1.410 |
| 18 | Fabienne Winkel (Neo) | NED | 40.62 | | +1.470 |
| 19 | Josien van der Wal (A2) | NED | 40.64 | | +1.490 |
| 20 | Monica Tuijp (Neo) | NED | 40.70 | | +1.550 |
| 21 | Anouk Karel (Neo) | NED | 40.76 | | +1.610 |
| 22 | Aveline Hylkema (Neo) | NED | 40.77 | | +1.620 |
| 23 | Ariane Smit (Neo) | NED | 40.82 | SB | +1.670 |
| 24 | Katarzyna Bachleda-Curuś (Senior) | POL | 40.89 | | +1.740 |
| 25 | Muriël Meijer (A2) | NED | 41.08 | | +1.930 |
| 26 | Katarzyna Wozniak (Senior) | POL | 41.17 | | +2.020 |
| 27 | Julia Noordenbos (Neo) | NED | 41.23 | | +2.080 |
| 28 | Jelena Peeters (Senior) | BEL | 41.28 | | +2.130 |
| 29 | Natasja Roest (Senior) | NED | 41.35 | | +2.200 |
| 30 | Sandra Dekker (A2) | NED | 41.36 | | +2.210 |
| 31 | Kelly Eijsink (Neo) | NED | 41.37 | PB | +2.220 |
| 32 | Anouk Sanders (Neo) | NED | 41.47 | | +2.320 |
| 33 | Martine Lilløy Bruun (Neo) | NOR | 41.52 | SB | +2.370 |
| 34 | Roza Blokker (Senior) | NED | 41.53 | | +2.380 |
| 35 | Willemijn Cnossen (Neo) | NED | 41.65 | | +2.500 |
| Jamie Schelling (Neo) | NED | 41.65 | | +2.500 |
| 37 | Rosanne Fischer (Neo) | NED | 41.66 | | +2.510 |
| 38 | Marit Steunenberg (Neo) | NED | 41.67 | | +2.520 |
| 39 | Paulien Verhaar (A1) | NED | 41.78 | | +2.630 |
| 40 | Linda Bortolotti (Neo) | ITA | 41.87 | SB | +2.720 |
| 41 | Pien Keulstra (Senior) | NED | 42.09 | | +2.940 |
| 42 | Magdalena Czyszczoń (Neo) | POL | 42.14 | SB | +2.990 |
| 43 | Loes Adegeest (Neo) | NED | 42.30 | SB | +3.150 |
| 44 | Marjolein van der Steen (Neo) | NED | 42.31 | | +3.160 |
| Sterre Jonkers (A2) | NED | 42.31 | | +3.160 |
| 46 | Tessa Sintenie (Neo) | NED | 42.40 | | +3.250 |
| 47 | Ellia Smeding (Neo) | GBR | 42.43 | | +3.280 |
| 48 | Eva Olde Hampsink (A1) | NED | 42.48 | PB | +3.330 |
| 49 | Eva Lagrange (Senior) | SWE | 42.54 | | +3.390 |
| 50 | Ramona Härdi (Neo) | SUI | 42.65 | SB | +3.500 |
| 51 | Renata Velthuijsen (Senior) | NED | 42.68 | | +3.530 |
| 52 | Laura Koldemeule (A1) | NED | 42.78 | | +3.630 |
| 53 | Dieke Hendriks (A1) | NED | 42.81 | | +3.660 |
| 54 | Anouk Timan (Neo) | NED | 43.19 | PB | +4.040 |
| 55 | Marieke Mulder (A1) | NED | 43.32 | | +4.170 |
| 56 | Gemma Cooper (Senior) | GBR | 43.94 | | +4.790 |