| 1 | Daniela Anschütz-Thoms (Senior) | GER | 40.49 | | - |
| 2 | Marrit Leenstra (A2) | NED | 40.50 | | +0.010 |
| 3 | Ingeborg Kroon (Neo) | NED | 40.59 | | +0.100 |
| 4 | Ireen Wüst (Neo) | NED | 40.83 | | +0.340 |
| 5 | Renate Groenewold (Senior) | NED | 40.90 | | +0.410 |
| 6 | Natasja Bruintjes (Neo) | NED | 40.97 | | +0.480 |
| 7 | Moniek Kleinsman (Senior) | NED | 41.00 | SB | +0.510 |
| 8 | Wieteke Cramer (Senior) | NED | 41.00 | | +0.510 |
| 9 | Paulien van Deutekom (Senior) | NED | 41.02 | | +0.530 |
| 10 | Tessa.86 van Dijk (Neo) | NED | 41.22 | | +0.730 |
| 11 | Jorien Voorhuis (Neo) | NED | 41.46 | | +0.970 |
| 12 | Sanne Delfgou (Neo) | NED | 41.54 | | +1.050 |
| 13 | Mayon Kuipers (Neo) | NED | 41.74 | | +1.250 |
| 14 | Ellen Hazelaar (Neo) | NED | 41.85 | | +1.360 |
| 15 | Mayke Nagtegaal (Neo) | NED | 41.89 | | +1.400 |
| 16 | Jorieke van der Geest (A1) | NED | 41.98 | | +1.490 |
| 17 | Anna Rokita (Neo) | AUT | 42.09 | | +1.600 |
| Annouk van der Weijden (Neo) | NED | 42.09 | | +1.600 |
| 19 | Maaike Elfferich (Neo) | NED | 42.37 | | +1.880 |
| 20 | Helen van Goozen (Senior) | NED | 42.47 | | +1.980 |
| 21 | Cindy Vergeer (Neo) | NED | 42.50 | | +2.010 |
| 22 | Karin Kalker (Neo) | NED | 42.51 | | +2.020 |
| 23 | Roxanne van Hemert (B2) | NED | 42.63 | | +2.140 |
| 24 | Foske Tamar van der Wal (Neo) | NED | 42.64 | | +2.150 |
| 25 | Kimberly van den Berg (Neo) | NED | 43.39 | | +2.900 |
| 26 | Chantal van Marle (Neo) | NED | 43.71 | | +3.220 |
| 27 | Cerise Tersteeg (C2) | NED | 45.57 | | +5.080 |
| 28 | Anne Spaargaren (A1) | NED | 46.29 | | +5.800 |
| 29 | Lotte van Dieren (Neo) | NED | 46.91 | | +6.420 |
| DNS | Maija Yli-Ojanperä (A1) | FIN | DNS | | - |