| 1 | Luisa Dehs (D1) | GER | 46.21 | SB | - |
| 2 | Maya Sophie Fleischer (D2) | GER | 46.25 | | +0.040 |
| 3 | Lucy Ludwig (D2) | GER | 46.37 | PB | +0.160 |
| 4 | Zoey Trant (D1) | GER | 47.16 | SB | +0.950 |
| 5 | Charlotte Heß (D1) | GER | 47.49 | | +1.280 |
| 6 | Rosa Anhalt (D2) | GER | 47.57 | | +1.360 |
| 7 | Lysann Licht (D2) | GER | 48.00 | SB | +1.790 |
| 8 | Elisabeth Schweizer-Theodor (D2) | GER | 48.20 | SB | +1.990 |
| 9 | Zoe Schmidt (D2) | GER | 48.93 | SB | +2.720 |
| 10 | Leonie Zinssmann (D1) | GER | 49.10 | | +2.890 |
| 11 | Mia Lara Thoms (D2) | GER | 49.12 | | +2.910 |
| 12 | Jule Neuy (D2) | GER | 49.21 | SB | +3.000 |
| 13 | Nele Wender-Krug (D1) | GER | 49.26 | SB | +3.050 |
| 14 | Amelie Sophia Roßner (D2) | GER | 50.44 | PB | +4.230 |
| 15 | Adele Weißflog (D1) | GER | 51.29 | SB | +5.080 |
| 16 | Aljona Voronkov (D1) | GER | 52.50 | SB | +6.290 |
| 17 | Constanze-Marie Mädler (D1) | GER | 52.91 | SB | +6.700 |
| 18 | Lisa Yang Benkendorf (D1) | GER | 53.06 | | +6.850 |
| 19 | Tamara Braun (D2) | GER | 58.44 | | +12.230 |