| 1 | Jolanda Meier (Neo) | NED | 43.54 | PB | - |
| 2 | Ruth Balvert (C2) | NED | 43.87 | | +0.330 |
| 3 | Marijn Lugt (C2) | NED | 44.08 | PB | +0.541 |
| 4 | Joline Boere (B2) | NED | 44.61 | | +1.073 |
| 5 | Anouk Beursgens (C2) | NED | 44.68 | | +1.146 |
| 6 | Amy Cornelissen (D2) | NED | 45.48 | SB | +1.946 |
| 7 | Wendy van de Ham (M40) | NED | 46.44 | SB | +2.902 |
| 8 | Quinty Ton (B2) | NED | 46.46 | | +2.920 |
| 9 | Ine Bonthuis (C2) | NED | 46.47 | | +2.933 |
| 10 | Karin van der Sar (M40) | NED | 46.80 | SB | +3.258 |
| 11 | Zoë Salemi (C1) | NED | 47.64 | | +4.106 |
| 12 | Linda Krikke (M40) | NED | 47.65 | PB | +4.114 |
| 13 | Amber Stommels (C2) | NED | 47.92 | SB | +4.386 |
| 14 | Sophia van de Laan (B1) | NED | 48.44 | | +4.904 |
| 15 | Tyche Wamsteker (D2) | NED | 48.63 | | +5.089 |
| 16 | Jitske Verschuur (C2) | NED | 49.01 | SB | +5.468 |
| 17 | Vera van Straten (C2) | NED | 49.18 | PB | +5.643 |
| 18 | Wieke Leenhouts (C1) | NED | 49.66 | SB | +6.119 |
| 19 | Rianne Coljé (C1) | NED | 49.96 | SB | +6.422 |
| 20 | Jacqueline van Schajik (M45) | NED | 50.72 | PB | +7.179 |
| 21 | Britney Pieterse (B1) | NED | 50.87 | SB | +7.334 |
| 22 | Marie Andel Wiersma-Talsma (M45) | NED | 51.29 | SB | +7.753 |
| 23 | Milou Middelkoop (C1) | NED | 51.30 | SB | +7.764 |
| 24 | Floor van Mourik (D2) | NED | 52.11 | SB | +8.574 |
| 25 | Bente Borsen (A1) | NED | 52.99 | SB | +9.453 |
| 26 | Iris Visser (C1) | NED | 53.11 | SB | +9.570 |
| 27 | Madelief Middelweerd (D2) | NED | 54.25 | | +10.710 |
| 28 | Marlijn van Aanhold (D2) | NED | 54.44 | SB | +10.900 |
| 29 | Lotte Maaijen (D2) | NED | 54.48 | SB | +10.943 |
| 30 | Marleen.02 van Straten (D2) | NED | 56.50 | SB | +12.959 |
| 31 | Juliëtte van der Ham (D2) | NED | 56.63 | PB | +13.089 |
| 32 | Anne de Vries (D1) | NED | 59.04 | SB | +15.498 |
| 33 | Ilva van Schaik (D2) | NED | 1:00.32 | | +16.784 |
| 34 | Eveline Zijderveld (C1) | NED | 1:05.51 | SB | +21.973 |
| DNS | Quinty van Klink (C2) | NED | DNS | | - |