| 1 | Ireen Wüst (Neo) | NED | 39.99 | | - |
| 2 | Paulien van Deutekom (Senior) | NED | 40.57 | | +0.580 |
| 3 | Mandy Groot (B1) | NED | 41.68 | | +1.690 |
| 4 | Jessica Baars (A2) | NED | 42.40 | | +2.410 |
| 5 | Hanne Tersmette (Neo) | NED | 45.68 | | +5.690 |
| 6 | Henriëtte Goede-Hartog (M40) | NED | 45.78 | | +5.790 |
| Roos van den Bos (B2) | NED | 45.78 | | +5.790 |
| 8 | Cynthia Baars (Neo) | NED | 45.88 | PB | +5.890 |
| 9 | Corina de Vrieze (Senior) | NED | 47.93 | | +7.940 |
| 10 | Suzanne Beets (B1) | NED | 48.08 | | +8.090 |
| 11 | Eva Peters (B2) | NED | 48.67 | | +8.680 |
| 12 | Bernadette Jeremiasse (B2) | NED | 48.98 | | +8.990 |
| 13 | Manon Floris (D2) | NED | 50.48 | | +10.490 |
| 14 | Louise van der Hijden (Senior) | NED | 51.87 | | +11.880 |
| 15 | Anne van Leeuwen (C1) | NED | 53.17 | | +13.180 |
| 16 | Xanthe Jeremiasse (D2) | NED | 53.61 | | +13.620 |
| 17 | Amber Mollee (B2) | NED | 53.93 | | +13.940 |
| 18 | Rosa Steffens (D2) | NED | 54.19 | | +14.200 |
| 19 | Katinka Dekker (C2) | NED | 54.47 | PB | +14.480 |
| 20 | Stephanie Zwart (A1) | NED | 56.04 | PB | +16.050 |
| 21 | Marloes Jongewaard (A1) | NED | 57.88 | SB | +17.890 |
| 22 | Maxine Gorter (C1) | NED | 59.29 | | +19.300 |
| 23 | Yvonne Kuin (M45) | NED | 1:01.10 | | +21.110 |
| 24 | Tamara Danser (C2) | NED | 1:02.53 | SB | +22.540 |
| 25 | Eline Neeft (C1) | NED | 1:04.63 | | +24.640 |