| 1 | Marjolein van der Steen (A2) | NED | 41.38 | SB | - |
| 2 | Janetta Tolsma (Neo) | NED | 41.79 | | +0.410 |
| Anne Marthe de Heer (Neo) | NED | 41.79 | PB | +0.410 |
| 4 | Anne Ruyssenaars (B2) | NED | 42.06 | | +0.680 |
| 5 | Raluca Doina Ştef (Neo) | ROU | 42.41 | | +1.030 |
| 6 | Margreeth den Hertog (C2) | NED | 42.89 | PB | +1.510 |
| 7 | Lianne van Loon (C2) | NED | 43.51 | SB | +2.130 |
| 8 | Naomi van der Werf (B1) | NED | 43.53 | SB | +2.150 |
| 9 | Suzanne Hoogendoorn (Senior) | NED | 44.06 | SB | +2.680 |
| 10 | Marloes Westerhof (M30) | NED | 44.12 | SB | +2.740 |
| 11 | Katja Lutz (Neo) | SUI | 44.13 | | +2.750 |
| 12 | Iris Kroeze (C2) | NED | 44.52 | | +3.140 |
| 13 | Marijn Lugt (C1) | NED | 44.70 | SB | +3.320 |
| 14 | Joyce Wolters (A2) | NED | 45.32 | | +3.940 |
| 15 | Myrthe Drogtrop (A1) | NED | 45.53 | SB | +4.150 |
| 16 | Ruth Balvert (C1) | NED | 45.89 | SB | +4.510 |
| 17 | Ine Bonthuis (C1) | NED | 47.19 | SB | +5.810 |
| 18 | Simone Veenstra (C1) | NED | 47.35 | SB | +5.970 |
| 19 | Sytske van Ballegooijen (D2) | NED | 47.63 | PB | +6.250 |
| 20 | Iris Veenstra (C2) | NED | 48.55 | PB | +7.170 |
| 21 | Tonnie Vinke (B2) | NED | 48.82 | PB | +7.440 |
| 22 | Vera van Ditshuizen (D1) | NED | 49.53 | SB | +8.150 |
| 23 | Isa Douma (D2) | NED | 50.90 | SB | +9.520 |
| 24 | Marieke Siemonsma (M60) | NED | 1:01.48 | PB | +20.100 |
| DQ | Annemee Gerritsen (B2) | NED | DQ | | - |