| 1 | Arthur Knibbe (Senior) | NED | 42.94 | PB | - |
| 2 | Djim Casander (Senior) | NED | 43.00 | | +0.060 |
| 3 | Tyan Cherepuschak (Neo) | CAN | 43.53 | PB | +0.590 |
| 4 | Roel van den Akker (Neo) | NED | 43.63 | PB | +0.690 |
| 5 | Martijn van der Zwaard (Senior) | NED | 43.70 | PB | +0.760 |
| 6 | Daniël Kievit (A2) | NED | 43.72 | PB | +0.780 |
| 7 | Sijbe Weening (Neo) | NED | 43.77 | SB | +0.830 |
| 8 | Lucas van der Sprong (Neo) | NED | 43.86 | PB | +0.920 |
| 9 | Martijn Cox (Neo) | NED | 44.32 | PB | +1.380 |
| 10 | Jack Bakker (Neo) | NED | 44.35 | PB | +1.410 |
| 11 | Boris Muller (Neo) | NED | 44.78 | PB | +1.840 |
| 12 | Dirk Grimm (Neo) | NED | 45.01 | PB | +2.070 |
| 13 | Jeroen Cox (Neo) | NED | 45.27 | PB | +2.330 |
| 14 | Jordy Krap (Neo) | NED | 45.51 | | +2.570 |
| 15 | Daan Pluister (Senior) | NED | 45.91 | PB | +2.970 |
| 16 | Hugo Hulleman (Senior) | NED | 46.11 | | +3.170 |
| 17 | Boris de Groot (Senior) | NED | 46.27 | SB | +3.330 |
| 18 | Friso Rip (Senior) | NED | 46.41 | PB | +3.470 |
| 19 | Daniel Jacobs (Neo) | NED | 46.57 | SB | +3.630 |
| 20 | Wouter Kelderman (Senior) | NED | 47.79 | SB | +4.850 |
| 21 | Sven Paternotte (Neo) | NED | 48.45 | SB | +5.510 |
| 22 | Sung Hoon Cho (Senior) | NED | 48.59 | PB | +5.650 |
| 23 | Jesper Kussendrager (Neo) | NED | 48.65 | | +5.710 |
| 24 | Colin Koenderink (Neo) | NED | 48.92 | | +5.980 |
| 25 | Lars Huiting (Neo) | NED | 49.02 | | +6.080 |
| 26 | Ewout Vos (n/a) | NED | 49.27 | SB | +6.330 |
| 27 | Berry ten Hoeve (Neo) | NED | 49.42 | SB | +6.480 |
| 28 | Rense Uitenbroek (Senior) | NED | 58.78 | PB | +15.840 |
| 29 | Rogier Venderbosch (Senior) | NED | 1:02.96 | | +20.020 |
| DNS | Luuk van der Vlerk (Neo) | NED | DNS | | - |