| 1 | Jeike Juk (Neo) | NED | 42.08 | PB | - |
| 2 | Tessa Huttenhuis (A2) | NED | 42.58 | SB | +0.500 |
| 3 | Maike Kroes (Neo) | NED | 43.04 | PB | +0.960 |
| 4 | Maaike van der Horst (Neo) | NED | 43.35 | PB | +1.270 |
| 5 | Hanna Jonker (Neo) | NED | 43.52 | PB | +1.440 |
| 6 | Anouk van Westerhoven (Senior) | NED | 43.71 | PB | +1.630 |
| 7 | Lotte Elfferich (Neo) | NED | 43.90 | SB | +1.820 |
| 8 | Esmee Messemaker (Neo) | NED | 44.09 | SB | +2.010 |
| 9 | Willemijn Cnossen (Senior) | NED | 44.15 | SB | +2.070 |
| Linda Pulles (Neo) | NED | 44.15 | SB | +2.070 |
| 11 | Eline Stubert (Senior) | NED | 44.71 | SB | +2.630 |
| 12 | Iris Smokers (Neo) | NED | 45.09 | SB | +3.010 |
| 13 | Allessandra Primavera (A2) | NED | 45.10 | PB | +3.020 |
| 14 | Lisa van Rijn (Senior) | NED | 45.32 | SB | +3.240 |
| 15 | Viviënne de Jong (Neo) | NED | 45.59 | PB | +3.510 |
| 16 | Emma Hulshof (Senior) | NED | 45.61 | | +3.530 |
| 17 | Frederieke Jansen (Senior) | NED | 45.71 | PB | +3.630 |
| 18 | Isa Groenewoud (A2) | NED | 45.75 | SB | +3.670 |
| 19 | Ellemijn Vernhout (Neo) | NED | 46.98 | SB | +4.900 |
| 20 | Kaija Berendschot (Neo) | NED | 47.03 | PB | +4.950 |
| Else Dekker (Senior) | NED | 47.03 | PB | +4.950 |
| 22 | Fenna Visser (Neo) | NED | 47.08 | PB | +5.000 |
| 23 | Deborah Veerman (Senior) | NED | 47.10 | PB | +5.020 |
| 24 | Carlijn Aarsen (A2) | NED | 47.45 | PB | +5.370 |
| 25 | Fimke Anna Giliam (Senior) | NED | 47.60 | PB | +5.520 |
| 26 | Amarins de Groot (Neo) | NED | 47.74 | | +5.660 |
| 27 | Tessa Roeven (Neo) | NED | 47.77 | PB | +5.690 |
| 28 | Yara van Wieringen (Neo) | NED | 47.99 | SB | +5.910 |
| 29 | Floor Hoevers (Neo) | NED | 48.18 | PB | +6.100 |
| 30 | Lisanne van der Werf (Senior) | NED | 49.49 | PB | +7.410 |
| 31 | Mirte Linthorst (Neo) | NED | 49.63 | SB | +7.550 |
| 32 | Laura Ammerlaan (Neo) | NED | 49.81 | PB | +7.730 |
| 33 | Leonie Peddemors (Senior) | NED | 50.18 | SB | +8.100 |
| 34 | Paula Teeuwen (Neo) | NED | 52.60 | | +10.520 |
| 35 | Esther Schrama (Senior) | NED | 54.59 | PB | +12.510 |
| 36 | Nienke Markensteijn (Senior) | NED | 56.22 | | +14.140 |
| 37 | Judith Termote (A2) | NED | 59.88 | SB | +17.800 |
| 38 | Katja Heidweiller (Senior) | NED | 1:00.14 | | +18.060 |
| 39 | Nanda Bloom (Neo) | NED | 1:00.61 | PB | +18.530 |
| 40 | Fabiënne Overbeek (n/a) | NED | 1:01.39 | PB | +19.310 |
| 41 | Jill Dissel (Neo) | NED | 1:02.49 | SB | +20.410 |
| RS | Jeske Hoogeboom (Senior) | NED | RS | | - |
| DNS | Bente Zijlstra (A2) | NED | DNS | | - |
| DQ | Myrthe Lagerwaard (Neo) | NED | DQ | | - |