| 1 | Tucker Fredricks (Senior) | USA | 35.76 | | - |
| 2 | Shani Davis (Senior) | USA | 35.92 | | +0.160 |
| 3 | Brent Aussprung (Neo) | USA | 35.95 | | +0.190 |
| 4 | Christopher Needham (Senior) | USA | 36.30 | | +0.540 |
| 5 | Matthew Plummer (Neo) | USA | 36.38 | | +0.620 |
| 6 | Nick Pearson (Senior) | USA | 36.61 | | +0.850 |
| 7 | Matthew Shanahan (A2) | USA | 36.67 | | +0.910 |
| 8 | Michael Blumel (Neo) | USA | 36.76 | | +1.000 |
| 9 | Mitchell Whitmore (A2) | USA | 36.86 | | +1.100 |
| 10 | Chad Hedrick (Senior) | USA | 36.99 | | +1.230 |
| Trevor Marsicano (Neo) | USA | 36.99 | | +1.230 |
| 12 | Clay James Cholewinski (Neo) | USA | 37.36 | | +1.600 |
| 13 | Ryan Bedford (Neo) | USA | 37.67 | | +1.910 |
| 14 | Daniel Beck (Neo) | USA | 38.02 | | +2.260 |
| 15 | JP Kepka (Senior) | USA | 38.20 | SB | +2.440 |
| 16 | Michael Stein-Stewart (Neo) | USA | 38.39 | | +2.630 |
| 17 | John Loquai (Senior) | USA | 38.44 | SB | +2.680 |
| 18 | Alexander Ochowicz (Neo) | USA | 38.52 | | +2.760 |
| 19 | Jeffrey Swider-Peltz (A2) | USA | 38.65 | | +2.890 |
| 20 | Colton Barrett (A1) | USA | 38.99 | | +3.230 |
| 21 | Joshua Wood (Senior) | USA | 39.20 | | +3.440 |
| 22 | Lee Eckert (Neo) | USA | 39.45 | | +3.690 |
| 23 | Buddy Gurr (Neo) | USA | 40.72 | | +4.960 |
| 24 | Nathaniel Defranco (Neo) | USA | 40.77 | | +5.010 |
| 25 | Charles Stangl (B2) | USA | 40.81 | | +5.050 |
| 26 | Nathan Tonkinson (B1) | USA | 41.72 | | +5.960 |
| 27 | Matthew Dahlmann (M50) | USA | 41.94 | | +6.180 |
| 28 | Joseph Blumel (B2) | USA | 41.95 | | +6.190 |
| 29 | Erik Ducker (B2) | USA | 42.95 | | +7.190 |
| 30 | Aron Chlebecek (B2) | USA | 42.97 | | +7.210 |
| 31 | Ryan Lent (Senior) | USA | 43.28 | | +7.520 |
| 32 | Olusegun Sijuwade (M45) | USA | 43.98 | | +8.220 |
| 33 | Richard Oudshoorn (Neo) | NED | 44.50 | | +8.740 |
| 34 | Matthew Backstrom (C2) | USA | 44.91 | | +9.150 |
| 35 | Nathan Shami (A1) | CAN | 45.49 | | +9.730 |
| 36 | Steven Penland (n/a) | USA | 50.50 | | +14.740 |
| 37 | Nathan Miller (D2) | USA | 51.06 | | +15.300 |
| 38 | Myron Yencha (M45) | USA | 53.20 | | +17.440 |
| 39 | Bren Piper (A1) | CAN | 53.33 | | +17.570 |
| 40 | Tyler Oliver (D1) | USA | 1:03.97 | SB | +28.210 |
| 41 | Chris Biocchi (A1) | CAN | 1:04.24 | | +28.480 |
| DQ | Charles Mahoney (Senior) | USA | DQ | | - |
| DNF | Alexander Mark (A2) | USA | DNF | | - |
| DNF | John Fine (Neo) | USA | DNF | | - |
| DNF | Alexander Ortiz (A2) | USA | DNF | | - |