| 1 | Brittany Bowe (Senior) | USA | 38.21 | | - |
| 2 | Sugar Todd (Senior) | USA | 38.72 | | +0.510 |
| 3 | Yu-Ting Huang (Senior) | TPE | 39.16 | NR | +0.950 |
| 4 | Paige Schwartzburg (Senior) | USA | 39.60 | | +1.390 |
| 5 | Rebekah Bradford-Plath (M35) | USA | 39.81 | NRM | +1.600 |
| 6 | Maria Victoria Rodriguez (Senior) | ARG | 40.46 | NR | +2.250 |
| 7 | Franchesca Pasquarella (Neo) | USA | 40.53 | | +2.320 |
| 8 | Jenni Kukkonen (Senior) | FIN | 40.94 | | +2.730 |
| 9 | Corinne Stoddard (B1) | USA | 41.17 | | +2.960 |
| 10 | Esther Munoz (Neo) | USA | 41.69 | | +3.480 |
| 11 | Mariah Richardson (Senior) | USA | 41.99 | | +3.780 |
| 12 | Jamie Lindquist-Nielson (A2) | USA | 42.29 | | +4.080 |
| 13 | Lindsey Woodbury (C2) | USA | 43.52 | | +5.310 |
| 14 | Ying-Chu Chen (Neo) | TPE | 44.19 | | +5.980 |
| 15 | Krissa Miller (M30) | USA | 44.54 | | +6.330 |
| 16 | Valeria Sarbu (M40) | USA | 51.66 | SB | +13.450 |
| 17 | Jocylyn Teece (E2) | USA | 1:01.63 | | +23.420 |