| 1 | Anna Quinn (Neo) | USA | 41.47 | | - |
| 2 | Sara Rehklau (Senior) | USA | 42.24 | | +0.770 |
| 3 | Sarah Warren (Senior) | USA | 42.33 | | +0.860 |
| 4 | Piper Yde (C2) | USA | 43.25 | | +1.780 |
| 5 | Sydney-Yu Terpening (A2) | USA | 43.76 | | +2.290 |
| 6 | Audrey Steffes (B2) | USA | 44.76 | | +3.290 |
| 7 | Mackenzie Neuendorf (Neo) | USA | 45.11 | | +3.640 |
| 8 | Brianne Molenda (B2) | USA | 45.25 | | +3.780 |
| Natasa Gakovic (C2) | USA | 45.25 | | +3.780 |
| 10 | Aaralyn Mcgill (C2) | CAN | 45.45 | | +3.980 |
| 11 | Muriel Kennedy (Neo) | USA | 45.60 | | +4.130 |
| 12 | Mirae Mastrolonardo (Neo) | USA | 45.82 | SB | +4.350 |
| 13 | Claire Kudlata (C2) | USA | 46.62 | | +5.150 |
| 14 | Rachel Mallard (B1) | CAN | 46.69 | | +5.220 |
| 15 | Brielle Durham (C2) | CAN | 46.78 | | +5.310 |
| 16 | Hayley Murray (B2) | CAN | 47.10 | SB | +5.630 |
| 17 | Barbara Johnson (M65) | USA | 47.64 | NRM | +6.170 |
| 18 | Sarah Murray (C2) | CAN | 47.67 | SB | +6.200 |
| 19 | Daria Vogt (B1) | CAN | 47.69 | SB | +6.220 |
| 20 | Catherine Girard (B2) | CAN | 47.71 | SB | +6.240 |
| 21 | Juliette Vaillancourt (B2) | CAN | 47.79 | SB | +6.320 |
| 22 | Jillian Reeves (C1) | CAN | 47.82 | PB | +6.350 |
| 23 | Emily Wood (B1) | CAN | 48.60 | | +7.130 |
| 24 | Heidi Beck (Senior) | CAN | 49.26 | SB | +7.790 |
| 25 | Deb Kohlmann (M35) | USA | 50.11 | SB | +8.640 |
| 26 | Stella Bickham-Okoniewski (D1) | USA | 50.80 | | +9.330 |
| 27 | Grace Hansen (n/a) | CAN | 55.70 | SB | +14.230 |
| 28 | Kaelan Joliffe (B2) | CAN | 1:06.18 | | +24.710 |
| 29 | Juliet Javorsky (E2) | USA | 1:08.62 | SB | +27.150 |