| 1 | Brittany Bowe (Senior) | USA | 37.93 | | - |
| 2 | Kimi Goetz (Senior) | USA | 38.08 | | +0.150 |
| 3 | Chrysta Rands-Evans (Senior) | USA | 38.79 | | +0.860 |
| 4 | McKenzie Browne (Senior) | USA | 38.99 | | +1.060 |
| 5 | Sarah Warren (Senior) | USA | 39.25 | SB | +1.320 |
| 6 | Maria Victoria Rodriguez (Senior) | ARG | 39.60 | | +1.670 |
| 7 | Blair Cruikshank (Senior) | USA | 40.10 | | +2.170 |
| 8 | Anna Quinn (Senior) | USA | 40.75 | SB | +2.820 |
| 9 | Piper Yde (Neo) | USA | 40.79 | SB | +2.860 |
| 10 | Libby Williams (Neo) | USA | 41.10 | | +3.170 |
| 11 | Emma de Bock (B1) | USA | 41.53 | SB | +3.600 |
| 12 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.64 | | +3.710 |
| 13 | Jenni Kukkonen (Senior) | FIN | 42.35 | | +4.420 |
| 14 | Amy Acosta (Neo) | ECU | 42.40 | NR | +4.470 |
| 15 | Shruti Kotwal (M30) | IND | 42.61 | | +4.680 |
| 16 | Karen Siatama (A2) | COL | 42.72 | | +4.790 |
| 17 | Isabela Vargas Rodriguez (A1) | COL | 42.96 | | +5.030 |
| 18 | Evie Forrer (B1) | USA | 43.67 | PB | +5.740 |
| 19 | Cynthia Jennings (Senior) | USA | 44.10 | SB | +6.170 |
| 20 | Natalie Rodrigue-Hasselback (M50) | USA | 45.30 | | +7.370 |
| 21 | Erin Jackson (Senior) | USA | 46.74 | | +8.810 |
| 22 | Barbara Johnson (M70) | USA | 49.01 | | +11.080 |
| 23 | Kathy Feinberg (M60) | USA | 58.37 | | +20.440 |