| 1 | Sarah Warren (Senior) | USA | 39.12 | SB | - |
| 2 | Maria Victoria Rodriguez (Senior) | ARG | 39.34 | SB | +0.220 |
| 3 | Anna Quinn (Senior) | USA | 41.13 | SB | +2.010 |
| 4 | Giorgia Birkeland (Senior) | USA | 41.36 | SB | +2.240 |
| Marley Soldan (A2) | USA | 41.36 | SB | +2.240 |
| 6 | Libby Williams (Neo) | USA | 41.40 | SB | +2.280 |
| 7 | Abigél Mercs (Neo) | HUN | 42.01 | PB | +2.890 |
| 8 | Ilsa Melin Sandvig Shobe (Senior) | USA | 42.20 | SB | +3.080 |
| 9 | Natalia Valencia (Senior) | COL | 42.29 | SB | +3.170 |
| 10 | Amy Acosta (Senior) | ECU | 42.38 | SB | +3.260 |
| 11 | Jenni Kukkonen (M35) | FIN | 42.46 | SB | +3.340 |
| 12 | Karen Lizeth Siatama Valencia (Neo) | COL | 42.49 | SB | +3.370 |
| 13 | Keeley Whitcomb (A1) | USA | 42.86 | SB | +3.740 |
| 14 | Leah Choi (A1) | USA | 42.94 | PB | +3.820 |
| 15 | Larissa Paes (M30) | BRA | 42.95 | SB | +3.830 |
| 16 | Shruti Kotwal (M30) | IND | 43.00 | SB | +3.880 |
| 17 | Natascha Lindenskov (Neo) | DEN | 43.01 | PB | +3.890 |
| 18 | Samantha Scheffler (Senior) | NED | 43.05 | SB | +3.930 |
| 19 | Ting Xuan Gu (B2) | TPE | 43.59 | NRJ | +4.470 |
| 20 | Ranja Ezzi (M40) | SWE | 43.62 | SB | +4.500 |
| 21 | Cynthia Jennings (Senior) | USA | 44.35 | SB | +5.230 |
| 22 | Kaitlyn Lee (B2) | USA | 44.50 | PB | +5.380 |
| Ana KImber (Senior) | USA | 44.50 | PB | +5.380 |
| 24 | Elisha Lee (C2) | USA | 44.95 | SB | +5.830 |
| 25 | Aileen Oh (C2) | USA | 45.46 | PB | +6.340 |
| 26 | Allison La (C2) | USA | 45.53 | SB | +6.410 |
| 27 | Chloe Kim (D2) | USA | 46.28 | PB | +7.160 |
| 28 | Natalie Rodrigue-Hasselback (M55) | USA | 47.31 | NRM | +8.190 |
| 29 | Juliette Song (C2) | USA | 48.75 | SB | +9.630 |
| 30 | Jeinn Oh (D1) | USA | 50.07 | PB | +10.950 |
| 31 | Sara Cushman (M50) | USA | 50.39 | PB | +11.270 |
| 32 | Shinn Dang (D2) | USA | 50.82 | PB | +11.700 |
| 33 | Nikita Quezon (D1) | USA | 51.04 | PB | +11.920 |
| 34 | Atira Meacham (D1) | USA | 55.50 | PB | +16.380 |
| 35 | Dasha Epperson (M40) | USA | 1:02.86 | SB | +23.740 |
| DNS | Julia Pereira de Vos (Neo) | BRA | DNS | | - |