| 1 | Maria Victoria Rodriguez (Senior) | ARG | 39.15 | SB | - |
| 2 | Anna Quinn (Senior) | USA | 40.92 | SB | +1.770 |
| 3 | Giorgia Birkeland (Senior) | USA | 41.27 | SB | +2.120 |
| 4 | Libby Williams (Neo) | USA | 41.29 | SB | +2.140 |
| 5 | Marley Soldan (A2) | USA | 41.73 | | +2.580 |
| 6 | Ilsa Melin Sandvig Shobe (Senior) | USA | 41.89 | SB | +2.740 |
| 7 | Abigél Mercs (Neo) | HUN | 42.05 | | +2.900 |
| 8 | Amy Acosta (Senior) | ECU | 42.21 | SB | +3.060 |
| 9 | Natalia Valencia (Senior) | COL | 42.31 | | +3.160 |
| 10 | Larissa Paes (M30) | BRA | 42.43 | NRM | +3.280 |
| 11 | Karen Lizeth Siatama Valencia (Neo) | COL | 42.52 | | +3.370 |
| 12 | Samantha Scheffler (Senior) | NED | 42.61 | PB | +3.460 |
| 13 | Ting Xuan Gu (B2) | TPE | 42.98 | NRJ | +3.830 |
| 14 | Natascha Lindenskov (Neo) | DEN | 43.15 | | +4.000 |
| 15 | Shruti Kotwal (M30) | IND | 43.26 | | +4.110 |
| 16 | Keeley Whitcomb (A1) | USA | 43.34 | | +4.190 |
| 17 | Ranja Ezzi (M40) | SWE | 43.40 | NRM | +4.250 |
| 18 | Elisha Lee (C2) | USA | 43.43 | PB | +4.280 |
| 19 | Cynthia Jennings (Senior) | USA | 43.45 | SB | +4.300 |
| 20 | Kaitlyn Lee (B2) | USA | 44.81 | | +5.660 |
| 21 | Ana KImber (Senior) | USA | 44.98 | | +5.830 |
| 22 | Allison La (C2) | USA | 45.40 | PB | +6.250 |
| 23 | Chloe Kim (D2) | USA | 46.73 | | +7.580 |
| 24 | Natalie Rodrigue-Hasselback (M55) | USA | 47.64 | | +8.490 |
| 25 | Sara Cushman (M50) | USA | 50.53 | | +11.380 |
| Jeinn Oh (D1) | USA | 50.53 | | +11.380 |
| 27 | Nikita Quezon (D1) | USA | 51.23 | | +12.080 |
| 28 | Shinn Dang (D2) | USA | 51.48 | | +12.330 |
| 29 | Atira Meacham (D1) | USA | 57.39 | | +18.240 |