| 1 | Nadezhda Morozova (Senior) | KAZ | 39.05 | SB | - |
| 2 | Kristina Silaeva (Senior) | KAZ | 39.11 | SB | +0.060 |
| 3 | Margarita Galiyeva (Neo) | KAZ | 39.50 | SB | +0.450 |
| 4 | Darja Vazhenina (Neo) | KAZ | 39.54 | PB | +0.490 |
| 5 | Luisa Gonzalez Salazar (Neo) | ESP | 39.63 | SB | +0.580 |
| 6 | Inessa Shumekova (Neo) | KAZ | 39.64 | SB | +0.590 |
| 7 | Carina Jagtøyen (Senior) | NOR | 39.77 | SB | +0.720 |
| 8 | Laethisia Schimek (Senior) | GER | 40.53 | SB | +1.480 |
| 9 | Josephine Heimerl (Senior) | GER | 40.54 | SB | +1.490 |
| 10 | Mathilde Pédronno (Senior) | FRA | 40.65 | NR | +1.600 |
| 11 | Arina Ilyachsehenko (Neo) | KAZ | 40.68 | PB | +1.630 |
| 12 | Sara Cabrera (Senior) | ESP | 41.03 | SB | +1.980 |
| 13 | Alena Lifatova (Neo) | KAZ | 41.11 | SB | +2.060 |
| 14 | Ona Rodriguez Cornejo (B2) | ESP | 41.25 | SB | +2.200 |
| 15 | Hanna Biró (Neo) | HUN | 41.61 | SB | +2.560 |
| 16 | Camilla Zazzarini (B1) | ITA | 42.08 | SB | +3.030 |
| 17 | Katerina Kainova (Neo) | CZE | 42.10 | PB | +3.050 |
| 18 | Noemi Libralesso (A1) | ITA | 42.26 | SB | +3.210 |
| 19 | Sarah Rosner (B2) | AUT | 42.35 | | +3.300 |
| 20 | Villiina Suomalainen (Neo) | FIN | 42.37 | PB | +3.320 |
| 21 | Emily Tormen (A2) | ITA | 42.40 | PB | +3.350 |
| 22 | Nikol Nepokojova (Neo) | CZE | 42.42 | SB | +3.370 |
| 23 | Lucie Korvasova (Neo) | CZE | 42.57 | SB | +3.520 |
| 24 | Aurora Aiello (B1) | ITA | 42.64 | PB | +3.590 |
| 25 | Giorgia Franceschini (B2) | ITA | 42.87 | SB | +3.820 |
| 26 | Rebeka Vancsó (A1) | HUN | 42.96 | SB | +3.910 |
| 27 | Christl Anders (Senior) | GER | 43.00 | SB | +3.950 |
| 28 | Sofiia Khochyna (Neo) | UKR | 43.03 | | +3.980 |
| 29 | Valentina Mendoza Álvarez (Neo) | ESP | 43.09 | SB | +4.040 |
| 30 | Lilla Sándor (B2) | HUN | 43.26 | SB | +4.210 |
| 31 | Katerina Macháckova (Neo) | CZE | 43.31 | SB | +4.260 |
| 32 | Sheila Gómez Fernandez (Neo) | ESP | 43.59 | SB | +4.540 |
| 33 | Mireia Tellez (B2) | ESP | 43.96 | PB | +4.910 |
| Aubane Plouhinec (A2) | FRA | 43.96 | PB | +4.910 |
| 35 | Manon Fraboulet (Neo) | FRA | 44.10 | PB | +5.050 |
| 36 | Carmen Thurner (A2) | ITA | 44.21 | SB | +5.160 |
| 37 | Oliegtnis Mercado (B2) | ESP | 44.24 | PB | +5.190 |
| 38 | Monica Seoane (A1) | ESP | 44.28 | SB | +5.230 |
| 39 | Paula Espin (B1) | ESP | 44.35 | PB | +5.300 |
| 40 | Meri-Vuokko Hannula (A1) | FIN | 44.43 | SB | +5.380 |
| 41 | Kateryna Demydenko (C2) | AUT | 44.86 | PB | +5.810 |
| Zoja Onuskova (B1) | SVK | 44.86 | PB | +5.810 |
| 43 | Barbora Sábliková (C2) | CZE | 44.95 | SB | +5.900 |
| 44 | Sophia Kopecková (C1) | CZE | 45.15 | PB | +6.100 |
| 45 | Monika Macháckova (A1) | CZE | 45.21 | SB | +6.160 |
| 46 | Sofia Gerber Della Pietra (B1) | AUT | 45.33 | | +6.280 |
| 47 | Emilia Hopf (C2) | GER | 45.52 | | +6.470 |
| 48 | Oona Koivupuisto (B2) | FIN | 45.54 | PB | +6.490 |
| 49 | Marta Papoušková (C2) | CZE | 46.46 | PB | +7.410 |
| 50 | Maiwenn Julou (A1) | FRA | 47.56 | PB | +8.510 |
| 51 | Iiris Vuorisalo (B1) | FIN | 48.45 | PB | +9.400 |
| WDR | Giorgia Fusetto (A2) | ITA | WDR | | - |