| 1 | Anna Ostlender (Neo) | GER | 38.65 | SB | - |
| 2 | Sophie Warmuth (Neo) | GER | 38.94 | SB | +0.290 |
| 3 | Margarita Galiyeva (Neo) | KAZ | 39.47 | SB | +0.820 |
| 4 | Ellia Smeding (Senior) | GBR | 39.63 | | +0.980 |
| 5 | Kaitlyn McGregor (Senior) | SUI | 39.72 | SB | +1.070 |
| 6 | Darja Vazhenina (Neo) | KAZ | 39.77 | | +1.120 |
| 7 | Kristina Shumekova (A1) | KAZ | 39.82 | SB | +1.170 |
| 8 | Inessa Shumekova (Neo) | KAZ | 39.89 | | +1.240 |
| 9 | Mathilde Pédronno (Senior) | FRA | 40.17 | NR | +1.520 |
| 10 | Jeannine Rosner (A1) | AUT | 40.31 | SB | +1.660 |
| 11 | Martina Sáblíková (Senior) | CZE | 40.41 | SB | +1.760 |
| 12 | Alena Lifatova (Neo) | KAZ | 40.70 | PB | +2.050 |
| 13 | Laethisia Schimek (Senior) | GER | 40.92 | | +2.270 |
| 14 | Laura Kivioja (Neo) | FIN | 40.96 | SB | +2.310 |
| 15 | Anastasija Belovodova (A1) | KAZ | 41.03 | | +2.380 |
| 16 | Marija Degen (A2) | KAZ | 41.34 | SB | +2.690 |
| 17 | Alina Shumekova (A1) | KAZ | 41.49 | SB | +2.840 |
| 18 | Giorgia Fusetto (A2) | ITA | 41.58 | | +2.930 |
| 19 | Nikol Nepokojova (Neo) | CZE | 41.85 | SB | +3.200 |
| 20 | Camilla Zazzarini (B1) | ITA | 41.99 | SB | +3.340 |
| 21 | Giorgia Franceschini (B2) | ITA | 42.42 | | +3.770 |
| 22 | Villiina Suomalainen (Neo) | FIN | 42.53 | | +3.880 |
| 23 | Rebeka Vancsó (A1) | HUN | 42.55 | SB | +3.900 |
| 24 | Sarah Rosner (B2) | AUT | 42.67 | | +4.020 |
| 25 | Aurora Aiello (B1) | ITA | 42.73 | | +4.080 |
| 26 | Aurelia Mulsow (Neo) | GER | 43.12 | SB | +4.470 |
| 27 | Zuzana Kursova (Neo) | CZE | 43.33 | SB | +4.680 |
| 28 | Lilla Sándor (B2) | HUN | 43.55 | | +4.900 |
| 29 | Jesika Forstova (A2) | CZE | 43.60 | PB | +4.950 |
| 30 | Barbora Sábliková (C2) | CZE | 44.72 | SB | +6.070 |
| 31 | Sara Hluskova (B2) | CZE | 44.86 | | +6.210 |
| 32 | Sofia Gerber Della Pietra (B1) | AUT | 45.00 | PB | +6.350 |
| 33 | Emilia Hopf (C2) | GER | 45.06 | SB | +6.410 |
| 34 | Lena Mothes (C2) | GER | 45.42 | SB | +6.770 |
| 35 | Zoja Onuskova (B1) | SVK | 45.52 | | +6.870 |
| 36 | Monika Macháckova (A1) | CZE | 45.66 | | +7.010 |
| 37 | Sophia Kopecková (C1) | CZE | 45.80 | | +7.150 |
| 38 | Lilly Engelhardt (C2) | GER | 45.89 | PB | +7.240 |
| 39 | Oona Koivupuisto (B2) | FIN | 45.91 | | +7.260 |
| 40 | Kateryna Demydenko (C2) | AUT | 46.35 | | +7.700 |
| 41 | Marta Papoušková (C2) | CZE | 46.48 | | +7.830 |
| 42 | Milla Isokorpi (C2) | FIN | 47.34 | SB | +8.690 |
| Johanna Brückner (C2) | GER | 47.34 | SB | +8.690 |
| 44 | July Seifert (C2) | GER | 49.49 | SB | +10.840 |
| 45 | Kateřina Vystrčilová (A1) | CZE | 50.42 | | +11.770 |
| 46 | Sara Mothes (D1) | GER | 53.53 | PB | +14.880 |
| DQ | Nikola Zdráhalova (Senior) | CZE | DQ | | - |